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Xujin

Professor,

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Email: xujin@scut.edu.cn

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About

Education

2002-2008                     Ph.D.                   National University of Singapore, Institute of Molecular and Cell Biology

Admission Information

WorkExperience

2008-2017   Research Assistant/Research Associate            Hong Kong University of Science and Technology

2017-now                   Professor                                            South China University of Technology


Social Position

Research

  Our laboratory is dedicated to studying the composition, development, and functions of both traditional and non-traditional clearance systems in the body. We primarily leverage the unique advantages of the zebrafish model to conduct studies that are difficult or impossible to perform in mice, with the goal of filling gaps in existing research frameworks. Zebrafish embryos are produced in large numbers and are transparent, making them an excellent model for forward genetic screening, real-time in vivo imaging, and optical microsurgery.

By observing the behavior and roles of clearance-related cells in real time during zebrafish development, we aim to gain a clearer understanding of the interactions between these cells and other tissue cells. Additionally, by designing appropriate forward genetic screens, we can uncover the roles of previously unknown genes in novel biological processes. Our laboratory strives to conduct innovative research by capitalizing on the unique advantages of the zebrafish model.

Current research focuses include:

  1. Investigating new functions of scavenger endothelial cells.

  2. Studying the development and function of classical innate immune cells.


Open Course

Biochemistry and Molecular Biology, Cell Biology


Research Project

National key R&D Program of China, the National Natural Science Foundation of China, National Natural Science Foundation of China (NSFC)/Research Grants Council (RGC) Joint Research Scheme, Guangdong Basic and Applied Basic Research Foundation, Guangdong Science and Technology Plan Projects

Thesis

Representative Publications:

1.     Dai Y, Jiang Y, Cao C, Xu Y, Lai S, Zhu W, Gao M, Li F#, He S#, Xu J#. (2025) Scavenger endothelial cells alleviate tissue damage by engulfing toxic molecules derived from hemolysis. Proc Natl Acad Sci U S A. 2025 Feb 18;122(7):e2406794122. doi: 10.1073/pnas.2406794122. Epub 2025 Feb 11. (#Co-Corresponding Author)

2.     Yu T#, Chen J, Wang Y, Xu J#. (2024) The embryonic zebrafish brain is exclusively colonized by pu.1-dependent and lymphatic-independent population of microglia. Science Advances. 2024 Aug 30;10(35):eado0519. doi: 10.1126/sciadv.ado0519. (#Co-Corresponding Author)

3.     Chen J, Wang H, Wu S, Zhang A, Qiu Z, Huang P, Qu Y J, Xu J#. (2024) col1a2+ fibroblasts/muscle progenitors finetune xanthophore countershading by differentially expressing csf1a/1b in embryonic zebrafish. Science Advances. 2024 Apr 5;10(14):eadj9637. doi: 10.1126/sciadv.adj9637.

4.     Chen Q, Kou H, Demy L D, Liu W, Li J, Wen Z, Herbomel P, Huang Z#, Zhang W#, Xu J#. (2024) The different roles of V-ATPase a subunits in phagocytosis/endocytosis and autophagy. Autophagy. 2024 Jun 25:1-17. doi: 10.1080/15548627.2024.2366748. (#Co-Corresponding Author)

5.     Huang Z, Xu Y, Qiu Z, Jiang Y, Wu J, Lin Q, He S, Qu JY, Chen J#, Xu J#. (2024) Caudal hematopoietic tissue supports definitive erythrocytes via epoa and is dispensable for definitive neutrophils. J Genet Genomics.2024 Feb 13:S1673-8527(24)00030-4. doi: 10.1016/j.jgg.2024.02.001. (#Co-Corresponding Author)

6.     Dai Y, Wu S, Cao C, Xue R, Luo X, Wen Z#, Xu J#. (2022) Csf1rb regulates definitive hematopoiesis in zebrafish. Development. 2022 Aug 15;149(16):dev200534. doi: 10.1242/dev.200534. (#Co-Corresponding Author)

7.     Lai S, Kumari A, Liu J, Zhang Y, Zhang W, Yen K#, Xu J#. (2021) Chemical screening reveals Ronidazole is a superior pro-drug to Metronidazole for Nitroreductase-induced cell ablation system in zebrafish larvae. J Genet Genomics. 2021 Aug 16: S1673-8527(21)00246-0. doi: 10.1016/j.jgg.2021.07.015. (#Co-Corresponding Author)

8.     He S, Tian Y, Feng S, Wu Y, Shen X, Chen K, He Y, Sun Q, Li X, Xu J#, Wen Z#, Qu JY#. (2020) In vivo single-cell lineage tracing in zebrafish using high-resolution infrared laser-mediated gene induction microscopy. elife. 2020 Jan 6;9. pii: e52024. doi: 10.7554/eLife.52024. (#Co-Corresponding Author)

9.     He SC, Chen JH, Jiang YY, Wu Y, Zhu L, Jin W, Zhao CL, Yu T, Wang TN, Wu ST, Lin X, Qu JY, Wen ZL, Zhang WQ#, Xu J#. (2018) Adult Zebrafish Langerhans Cells Arise from Hematopoietic Stem/progenitor Cells. eLife. 2018 Jun 15; 7. (#Co-Corresponding Author)

10.  Xu J*, Wang TN*, Wu Y, Jin W, Wen ZL. (2016) Microglia Colonization of Developing Zebrafish Midbrain Is Promoted by Apoptotic Neuron and Lysophosphatidylcholine. Dev Cell. 2016 Jul 25; 38(2):214-22. (*equal contribution)

11.  Xu J*, Zhu L*, He S, Wu Y, Jin W, Yu T, Qu JY, Wen ZL. (2015) Temporal-Spatial Resolution Fate Mapping Reveals Distinct Origins for Embryonic and Adult Microglia in Zebrafish. Dev Cell. 2015 Sep 28; 34(6):632-41. (*equal contribution)

12.  Xu J, Du L, Wen ZL. (2012) Myelopoiesis during zebrafish early development. J Genet Genomics. 2012 Sep 20; 39(9):435-42.      (review)

13.  Du LS*, Xu J*, Li XL, Ma N, Liu YM, Peng JR, Osato M, Zhang WQ, and Wen ZL. (2011) Rumba and Haus3 are essential factors for the maintenance of hematopoietic stem/progenitor cells during zebrafish hematopoiesis. Development. 2011 Feb; 138(4): 619-29. (*equal contribution)

14.  Jin H*, Xu J*, and Wen ZL. (2007) Migratory path of definitive hematopoietic stem/progenitor cells during zebrafish development. Blood. 2007 Jun 15;109(12): 5208-14. (* equal contribution)


Other Publications:

1.     Xu, WH, Dong, JJ, Dai, YM, Zhao, Y, Qin, QW, Huang, XH#, Xu, J#, Huang, YH#. (2024) Establishment of an LMBV-infection model in zebrafish larvae and its application in studying virus-host interactions. Aquaculture. 2024 Dec 15; DOI10.1016/j.aquaculture.2024.741280. (#Co-Corresponding Author)

2.     Chen X, Li Y, Xu J, Cui Y, Wu Q, Yin H, Li Y, Gao C, Jiang L, Wang H, Wen Z, Yao Z, Wu Z. (2024) Styxl2 regulates de novo sarcomere assembly by binding to non-muscle myosin IIs and promoting their degradation. Elife. 2024 Jun 3;12:RP87434. doi: 10.7554/eLife.87434.

3.     Zhao S, Mo G, Wang Q, Xu J, Yu S, Huang Z, Liu W, Zhang W. (2024) Role of RB1 in neurodegenerative diseases: inhibition of post-mitotic neuronal apoptosis via Kmt5b. Cell Death Discov. 2024 Apr 18;10(1):182. doi: 10.1038/s41420-024-01955-y.

4.     Zhao S, Wang C, Luo H, Li F, Wang Q, Xu J, Huang Z, Liu W, Zhang W. (2024) A role for Retinoblastoma 1 in hindbrain morphogenesis by regulating GBX family. J Genet Genomics. 2024 Sep;51(9):900-910. doi: 10.1016/j.jgg.2024.03.008. Epub 2024 Apr 1.

5.     Zhang A, Lu J, Feng S, Yu H, Yu T, Zhao S, Chen K, Huang Z, Xu J, Qu JY, Wen Z. (2024) Fli1 acts in parallel with Pu.1 to control macrophage and neutrophil fate in zebrafish. J Genet Genomics. 2024 Mar;51(3):359-362. doi: 10.1016/j.jgg.2023.11.001. Epub 2023 Nov 8.

6.     Sun W, Wang M, Zhao J, Zhao S, Zhu W, Wu X, Li F, Liu W, Wang Z, Gao M, Zhang Y, Xu J, Zhang M, Wang Q, Wen Z, Shen J, Zhang W, Huang Z. (2023) Sulindac selectively induces autophagic apoptosis of GABAergic neurons and alters motor behaviour in zebrafish. Nat Commun. 2023 Sep 2;14(1):5351. doi: 10.1038/s41467-023-41114-y.

7.     Deng Y, Wang H, Liu X, Yuan H, Xu J, de Thé H, Zhou J, Zhu J. (2022) Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish. Elife. 2022 Oct 7;11:e80760. doi: 10.7554/eLife.80760.

8.     Lou L, Yu T, Dai Y, Zhao S, Feng S, Xu J, Wen Z. (2022) Mafba and Mafbb regulate microglial colonization of zebrafish brain via controlling chemotaxis receptor expression. Proc Natl Acad Sci U S A. 2022 Sep 27;119(39):e2203273119. doi: 10.1073/pnas.2203273119. Epub 2022 Sep 19.

9.     Wu L, Xue R, Chen J#, Xu J#. (2022) dock8 deficiency attenuates microglia colonization in early zebrafish larvae. Cell Death Discov. 2022 Aug 17;8(1):366. doi: 10.1038/s41420-022-01155-6. (#Co-Corresponding Author)

10.  Yang S, Ning G, Hou Y, Cao Y, Xu J, Wu J, Zhang T, Wang Q. (2022) Myoneurin regulates BMP signaling by competing with Ppm1a for Smad binding. iScience. 2022 May 30;25(6):104495. doi: 10.1016/j.isci.2022.104495.

11.  Xue R, Wang Y, Wang T, Lyu M, Mo G, Fan X, Li J, Yen K#, Yu S#, Liu Q#, Xu J#. (2021) Functional Verification of Novel ELMO1 Variants by Live Imaging in Zebrafish. Front Cell Dev Biol. 2021 Dec 21;9:723804. doi: 10.3389/fcell.2021.723804. eCollection 2021. (#Co-Corresponding Author)

12.  Wu M, Xu Y, Li J, Lian J, Chen Q, Meng P, Lu T, Xie H, Zhang W, Xu J, Zhang Y. (2021) Genetic and epigenetic orchestration of Gfi1aa-Lsd1-cebpa in zebrafish neutrophil development. Development. 2021 Sep 1; 148(17): dev199516. doi: 10.1242/dev.199516.

13.  Fang X, Xu S, Zhang Y, Xu J, Huang Z, Liu W, Wang S, Yen K, Zhang W. (2021) Asxl1 C-terminal mutation perturbs neutrophil differentiation in zebrafish. Leukemia.2021 Jan 22. doi: 10.1038/s41375-021-01121-8.

14.  Huang Z, Chen K, Chi Y, Jin H, Li L, Zhang W, Xu J, Zhang Y. (2021) Runx1 regulates zebrafish neutrophil maturation via synergistic interaction with c-Myb. J Biol Chem.2021 Jan 9:100272. doi: 10.1016/j.jbc.2021.100272.

15.  Wu S, Nguyen LTM, Pan H, Hassan S, Dai Y, Xu J, Wen Z. (2020) Two phenotypically and functionally distinct microglial populations in adult zebrafish. Sci Adv.2020 Nov 18;6(47):eabd1160. doi: 10.1126/sciadv.abd1160.

16.  He S, Xu J, Qu JY#, Wen Z#. (2020) Lightening the way of hematopoiesis: Infrared laser-mediated lineage tracing with high spatial-temporal resolution.Exp Hematol. 2020 May;85:3-7. doi: 10.1016/j.exphem.2020.04.009.     (Perspective)

17.  Xu M, Ye Y, Ye Z, Xu S, Liu W, Xu J, Zhang Y, Liu Q, Huang Z, Zhang W. (2020) Human BCR/ABL1 Induces Chronic Myeloid Leukemia-Like Disease in Zebrafish. Haematologica. 2020 Mar;105(3):674-686. doi: 10.3324/haematol.2019.215939.

18.  Jiang Y, Chen J, Xu J#. (2019) An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo. J Vis Exp. 2019 Sep 25; (151)

19.  Lin X, Wen Z#, Xu J#. (2019) Tissue-resident macrophages: from zebrafish to mouse. Blood Sci. 2019 Sep 17;1(1):57-60. doi: 10.1097/BS9.0000000000000013. eCollection 2019 Aug. (#Co-Corresponding Author)      (review)

20.  Lin X, Zhou Q, Zhao C, Lin G, Xu J, Wen Z. (2019) An Ectoderm-Derived Myeloid-like Cell Population Functions as Antigen Transporters for Langerhans Cells in Zebrafish Epidermis. Dev Cell. 2019 May 20;49(4):605-617.

21.  Jiang Y, Chen J, Yen K#, Xu J#. (2018) Ectopically Expressed IL-34 Can Efficiently Induce Macrophage Migration to the Liver in Zebrafish. Zebrafish. 2019 Feb 6. doi: 10.1089/zeb.2018.1685. (#Co-Corresponding Author)

22.  Wu S, Xue R, Hassan S, Nguyen TML, Wang T, Pan H, Xu J, Liu Q, Zhang W, Wen Z. (2018) Il34-Csf1r Pathway Regulates the Migration and Colonization of Microglial Precursors. Dev Cell. 2018 Sep 10;46(5):552-563.e4.

23.  Chi Y, Huang Z, Chen Q, Xiong X, Chen K, Xu J, Zhang Y, Zhang W. (2018) Loss of runx1 function results in B cell immunodeficiency but not T cell in adult zebrafish. Open Biol. 2018 Jul;8(7).

24.  Lian J, Chen J, Wang K, Zhao L, Meng P, Yang L, Wei J, Ma N, Xu J, Zhang W, Zhang Y. (2018) Alas1 is essential for neutrophil maturation in zebrafish. Haematologica. 2018 Nov;103(11):1785-1795.

25.  Zhang Z, Liu W, Zhao L, Huang Z, Chen X, Ma N, Xu J, Zhang W, Zhang Y. (2018) Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1. Development. 2018 Jan 8;145(1).

26.  Tian Y, Xu J, Feng S, He S, Zhao S, Zhu L, Jin W, Dai Y, Luo L, Qu JY, Wen Z. (2017) The first wave of T lymphopoiesis in zebrafish arises from aorta endothelium independent of hematopoietic stem cells. J Exp Med. 2017 Nov 6;214(11):3347-3360.

27.  Yu T, Guo W, Tian Y, Xu J, Chen J, Li L, Wen Z. (2017) Distinct regulatory networks control the development of macrophages of different origins in zebrafish. Blood. 2017 Jan 26;129(4):509-519.

28.  Jin H, Huang Z, Chi Y, Wu M, Zhou R, Zhao L, Xu J, Zhen F, Lan Y, Li L, Zhang W, Wen ZL, Zhang Y. (2016) cMyb acts in parallel and cooperatively with Cebp1 to regulate neutrophil maturation in zebrafish. Blood. 2016 Jul 21; 128(3):415-26.

29.  Wei Y, Xu J, Zhang W, Wen ZL, Liu F. (2016) RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish. Development. 2016 Jun 15; 143(12):2103-10.

30.  Zeng Y, Yan B, Xu J, Sun Q, He S, Jiang J, Wen ZL, Qu JY. (2014) In Vivo nonlinear optical imaging of immune responses: tissue injury and infection. Biophys J. 2014 Nov 18; 107(10):2436-43.

31.  Li X, Lan Y, Xu J, Zhang WQ, Wen ZL. (2012) SUMO1-activating enzyme subunit 1 is essential for the survival of hematopoietic stem/progenitor cells in zebrafish. Development.2012 Dec 1; 139(23):4321-9.

32.  Zeng Y, Xu J, Li D, Li L, Wen ZL, Qu JY. (2012) Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging. Opt Lett. 2012 Jul 1; 37(13):2490-2.

33.  Jin H, Li L, Xu J, Zhen F, Zhu L, Liu PP, Zhang MJ, Zhang WQ, Wen ZL. Runx1 regulates embryonic myeloid fate choice in zebrafish through a negative feedback loop inhibiting Pu.1 expression. Blood. 2012 May 31; 119(22):5239-49.

34.  Li L, Jin H, Xu J, Shi YQ, and Wen ZL. (2011) Irf8 regulates macrophage versus neutrophil fate during zebrafish primitive myelopoiesis. Blood.2011 Jan 27;117(4): 1359-69.

35.  Jin H, Sood R, Xu J, Zhen FH, Liu PP, andWen ZL. (2009) Definitive hematopoietic stem cells manifest distinct differentiation output in the zebrafish VDA and PBI.Development. 2009 Feb; 136(4): 647-54.

36.  Liu YM, Du LS, Osato M, Teo EH, Qian F, Jin H, Zhen FH, Xu J, Guo L, Huang HH, Chen J, Geisler R, Jiang YJ, Peng JR and Wen ZL. (2007) The zebrafish udu gene encodes a novel nuclear factor and is essential for primitive erythroid cell development. Blood.2007 Jul 1;110(1): 99-106.

37.  Qian F, Zhen FH, Xu J, Huang M, Li WY, and Wen ZL. (2007) Distinct functions for different scl isoforms in zebrafish primitive and definitive hematopoiesis. PLoS Biology. 2007 May; 5(5): e132.

38.  Jin H*, Xu J*, Qian F, Du LS, Tan CY, Lin ZX, Peng JR, and Wen ZL.(2006) The 5’ zebrafish scl promoter targets transcription to the brain, spinal cord, hematopoietic and endothelial progenitors. Developmental Dynamics. 2006 Jan; 235(1): 60-7. (*equal contribution)


Achievements

Patent

Honor

Software achievement