个人简介
教育经历
2002-2006 清华大学 化学工程系 化学工程与工艺 学士
2006-2011 清华大学 化学工程系 化学工程与技术（生物化工方向） 博士
招生信息
- 学术型博导,生物科学与工程学院,071000,生物学,生物化学与分子生物学
- 学术型硕导,生物科学与工程学院,071000,生物学,生物化学与分子生物学
- 专业学位硕导,086000,生物工程
- 专业学位硕导,105500
工作经历
- 2018~至今,华南理工大学 生物科学与工程学院
- 2011~2018,美国加州斯克里普斯研究所（The Scripps Research Institute） 化学系
社会、学会及学术兼职
教育部合成生物学与药物制备国际合作联合实验室副主任
《Journal of the American Chemical Society》、《Angewandte Chemie International Edition》等国际著名学术期刊审稿专家
研究领域
科研
科研兴趣：蛋白质工程、分子定向进化、基于非天然碱基对的遗传字母/遗传密码扩展、基于非天然核酸的遗传信息载体拓展/新型生物功能大分子开发、合成生物学、化学生物学、生物材料、大分子药物、新型检测技术、人工生命
开授课程
教学
本科生教学：生命科学与营养健康导论、生物科学与工程概论、合成生物学导论
科研项目
科研项目：
1、新型蛋白质进化技术和平台开发；
2、新型非天然碱基对/非天然核酸元件的合成；
3、非天然碱基对/非天然核酸工具酶（包括聚合酶）的蛋白质工程；
4、高效DNA/RNA/非天然碱基DNA/非天然核酸合成/扩增技术的开发；
5、非天然核酸分子的进化（SELEX）及基于非天然核酸分子的检测方法/大分子药物的开发；
6、基于非天然碱基对/非天然核酸的新型生物材料的开发；
7、基于非天然碱基对/非天然核酸的半合成人工生命的开发、优化和应用研究。
论文
- Hantao Luo, Yuhui Du, Leping Sun, Fangkai Ye, Jiezhao Ma, Xueting Wang, Yaxin Wang, Tingjian Chen,Recognition of unnatural base pairs by a eukaryotic DNA polymerase enables universal sequencing of an expanded genetic alphabet,Nucleic Acids Research,2026
- Zhang, Binliang;Du, Yuhui;Zhang, Jingxing;Ma, Xingyun;Qin, Yanjia;Tao, Rui;Luo, Minglan;Wu, Jing;Sun, Leping;Zhu, Gan;Luo, Hantao;Wen, Junlin;Xiong, Chenghe;Mei, Hui;Chen, Tingjian,A solid-phase enzymatic synthesis platform for the facile production of 2′-fluoroarabinonucleic acid (FANA) and chimeric XNA oligonucleotides using an evolved XNA polymerase,Nucleic Acids Research,2025
- Peng, Leli;Ye, Fangkai;Zhang, Ruimin;Du, Yuhui;Liu, Qiaoling;Zhang, Rujie;Zhang, Binliang;Chen, Tingjian,Rolling Circle Amplification with an Expanded Genetic Alphabet (ExRCA) Generates DNA Materials Incorporating Additional Building Blocks for Enhanced Programmable Functionalization,Journal of the American Chemical Society,2025
- Li, Xiaofeng;Shan, Xi;Chen, Jiadong;Zhu, Jun;Chen, Yang;Chen, Xueyi;Li, Shahao;Lu, Mengze;Du, Yuhui;Yin, Panchao;Chen, Tingjian;Sun, Taolin,Bioinspired DNA Plastics with Brick-and-Mortar Structure: Enhanced Toughness, Recyclability, and Degradability,Chem and Bio Engineering,2025
- Wu, Jing;Xiao, Ziqian;Du, Yuhui;Luo, Minglan;Zhang, Jiacheng;Guo, Pei;Chen, Zhipeng;Yang, Binyuan;Chen, Tingjian,Generation of Stable 2'-F/OMe-RNA Aptasensors for Bacillus cereus 5/B/6 Metallo-β-Lactamase,ACS Synthetic Biology,2025
- Du, Yuhui;Zheng, Xinqian;Ma, Yuan;Ge, Fangjun;Li, Qiuyan;Xiang, Hao;Chen, Xueyi;Zhou, Hai;Sun, Taolin;Chen, Tingjian,Preparation of Biomass-Derived DNA-Chitosan Polyelectrolyte Complex Hydrogels for Economical and Reliable DNA Preservation and Protein Immobilization,ACS Sustainable Chemistry and Engineering,2024
- Sun, Leping;Xiang, Yuming;Du, Yuhui;Wang, Yangming;Ma, Jiezhao;Wang, Yaxin;Wang, Xueting;Wang, Guangyuan;Chen, Tingjian,Template-independent synthesis and 3′-end labelling of 2′-modified oligonucleotides with terminal deoxynucleotidyl transferases,Nucleic Acids Research,2024
- Du, Yuhui;Qian, Chengqian;Li, Xianxin;Zheng, Xinqian;Huang, Shoucong;Yin, Zhiqiu*;Chen, Tingjian*;Pan, Li*,Unveiling intraspecific diversity and evolutionary dynamics of the foodborne pathogen Bacillus paranthracis s through high-quality pan-genome analysis,Current Research in Food Science,2024
- Wen, Chongzheng;Wang, Guangyuan;Yang, Lin;Chen, Tingjian;Liu, Haiping;Gong, Weimin,Structural Basis for C2'-methoxy Recognition by DNA Polymerases and Function Improvement,Journal of Molecular Biology,2024
- Guangyuan Wang, Yuhui Du, Tingjian Chen,Enzymatic Preparation of DNA with an Expanded Genetic Alphabet Using Terminal Deoxynucleotidyl Transferase and Its Applications,Synthetic Biology: Methods and Protocols,2024
- Du, Yuhui;Zou, Jinrong;Yin, Zhiqiu;Chen, Tingjian,Pan-Chromosome and Comparative Analysis of Agrobacterium fabrum Reveal Important Traits Concerning the Genetic Diversity, Evolutionary Dynamics, and Niche Adaptation of the Species,Microbiology Spectrum,2023
- Cao, Yijun;Bai, Jingsi;Zou, Jinrong;Du, Yuhui;Chen, Tingjian,One-Pot Enzymatic Preparation of Oligonucleotides with an Expanded Genetic Alphabet via Controlled Pause and Restart of Primer Extension: Making Unnatural Out of Natural,ACS Synthetic Biology,2023
- Qin, Yanjia;Ma, Xingyun;Tao, Rui;Du, Yuhui;Chen, Tingjian,Synthesis, Reverse Transcription, Replication, and Inter-Transcription of 2'-Modified Nucleic Acids with Evolved Thermophilic Polymerases: Efforts toward Multidimensional Expansion of the Central Dogma,ACS Synthetic Biology,2023
- Bai, Jingsi;Zou, Jinrong;Cao, Yijun;Du, Yuhui;Chen, Tingjian,Recognition of an Unnatural Base Pair by Tool Enzymes from Bacteriophages and Its Application in the Enzymatic Preparation of DNA with an Expanded Genetic Alphabet,ACS Synthetic Biology,2023
- Chen, Xueyi;Zhang, Rujie;Peng, Leli;Du, Yuhui;Chen, Tingjian,Bottlebrush DNA-Primed Rolling Circle Amplification for Sensitive Detection of Biomolecules,ACS Applied Nano Materials,2023
- Guo, Yuan-Yang;Zhang, Rujie;Huo, Bianbian;Wang, Luying;Yuan, Donghe;Bai, Jingsi;Wang, Honglei;Zhu, Gongming;Zhang, Xiguang;Zhu, Anlian;Chen, Tingjian;Li, Lingjun,Site-specific unnatural base excision via visible light,Chemical communications,2022
- Lu, Mengze;Liu, Fei;Tan, Rui;Xiao, Zhenhua;Dong, Xue-hui;Wang, Hui;Tang, Liqun;Chen, Tingjian;Wu, Zi Liang;Hong, Wei;Sun, Taolin,Phase-Separation-Induced Porous Hydrogels from Amphiphilic Triblock Copolymer with High Permeability and Mechanical Strength,Chemistry of Materials,2022
- Zhu, Gan;Song, Ping;Wu, Jing;Luo, Minglan;Chen, Zhipeng;Chen, Tingjian,Application of Nucleic Acid Frameworks in the Construction of Nanostructures and Cascade Biocatalysts: Recent Progress and Perspective,Frontiers in Bioengineering and Biotechnology,2022
- Wang, Guangyuan;He, Chuanping;Zou, Jinrong;Liu, Jiayun;Du, Yuhui;Chen, Tingjian,Enzymatic Synthesis of DNA with an Expanded Genetic Alphabet Using Terminal Deoxynucleotidyl Transferase,ACS Synthetic Biology,2022
- Wang Guangyuan;Du Yuhui;Ma Xingyun;Ye Fangkai;Qin Yanjia;Wang Yangming;Xiang Yuming;Tao Rui;Chen Tingjian,Thermophilic Nucleic Acid Polymerases and Their Application in Xenobiology,International Journal of Molecular Sciences,2022
- Sun Leping;Ma Xingyun;Zhang Binliang;Qin Yanjia;Ma Jiezhao;Du Yuhui;Chen Tingjian,From polymerase engineering to semi-synthetic life: artificial expansion of the central dogma,RSC Chemical Biology,2022
- Song Ping;Zhang Rujie;He Chuanping;Chen Tingjian,Transcription, Reverse Transcription, and Amplification of Backbone-Modified Nucleic Acids with Laboratory-Evolved Thermophilic DNA Polymerases.,Current protocols,2021
- Qian Shao, Tingjian Chen, Kai Sheng, Zhixia Liu, Zhuochen Zhang, Floyd E Romesberg,Selection of aptamers with large hydrophobic 2′-substituents,Journal of the American Chemical Society,2020
- Deepak Thirunavukarasu, Tingjian Chen, Zhixia Liu, Narupat Hongdilokkul, Floyd E Romesberg,Selection of 2′-fluoro-modified aptamers with optimized properties,Journal of the American Chemical Society,2017
- Zhixia Liu, Tingjian Chen, Floyd E Romesberg,Evolved polymerases facilitate selection of fully 2′-OMe-modified aptamers,Chemical Science,2017
- Tingjian Chen, Floyd E Romesberg,Enzymatic synthesis, amplification, and application of DNA with a functionalized backbone,Angewandte Chemie International Edition,2017
- Tingjian Chen, Floyd E Romesberg,Polymerase chain transcription: exponential synthesis of RNA and modified RNA,Journal of the American Chemical Society,2017
- Tingjian Chen, Floyd E Romesberg,A method for the exponential synthesis of RNA: introducing the polymerase chain transcription (PCT) reaction,Biochemistry,2017
- Tingjian Chen, Narupat Hongdilokkul, Zhixia Liu, Ramkrishna Adhikary, Shujian S Tsuen, Floyd E Romesberg,Evolution of thermophilic DNA polymerases for the recognition and amplification of C2ʹ-modified DNA,Nature chemistry,2016
- Tingjian Chen, Narupat Hongdilokkul, Zhixia Liu, Deepak Thirunavukarasu, Floyd E Romesberg,The expanding world of DNA and RNA,Current opinion in chemical biology,2016
- Ramkrishna Adhikary, Wayne Yu, Masayuki Oda, Ross C Walker, Tingjian Chen, Robyn L Stanfield, Ian A Wilson, Jörg Zimmermann, Floyd E Romesberg,Adaptive mutations alter antibody structure and dynamics during affinity maturation,Biochemistry,2015
- Denis A Malyshev, Kirandeep Dhami, Thomas Lavergne, Tingjian Chen, Nan Dai, Jeremy M Foster, Ivan R Corrêa, Floyd E Romesberg,A semi-synthetic organism with an expanded genetic alphabet,Nature,2014
- Tingjian Chen, Floyd E Romesberg,Directed polymerase evolution,FEBS letters,2014
- Tingjian Chen, Jianqing Wang, Lingli Zeng, Rizong Li, Jicong Li, Yilu Chen, Zhanglin Lin,Significant rewiring of the transcriptome and proteome of an Escherichia coli strain harboring a tailored exogenous global regulator IrrE,PLoS One,2012
- Tingjian Chen, Jianqing Wang, Rong Yang, Jicong Li, Min Lin, Zhanglin Lin,Laboratory-evolved mutants of an exogenous global regulator, IrrE from Deinococcus radiodurans, enhance stress tolerances of Escherichia coli,PLoS One,2011
- Chuan Ren, Tingjian Chen, Jingqing Zhang, Ling Liang, Zhanglin Lin,An evolved xylose transporter from Zymomonas mobilis enhances sugar transport in Escherichia coli,Microbial cell factories,2009
- Tingjian Chen, Jingqing Zhang, Ling Liang, Rong Yang, Zhanglin Lin,An in vivo, label-free quick assay for xylose transport in Escherichia coli,Analytical biochemistry,2009
- Yong Chen, Shuang Li, Tingjian Chen, Hui Hua, Zhanglin Lin,Random dissection to select for protein split sites and its application in protein fragment complementation,Protein Science,2009
出版专著和教材
专利
- 陈庭坚;汪杨铭;王光远;马杰钊;刘又萌,具有XNA合成及反转录活性的Bst DNA聚合酶突变株,2022116858093,2026-04-14
- 陈庭坚;毛怡馨;朱淦;梅隽彦;李函临;黄欣,一种2'-氟-2'-脱氧核苷-5'-三磷酸的酶促合成方法及其应用,2024119267145,2025-11-21
- 陈庭坚;张汝洁;彭乐丽;叶方开;陈薛亦;何传平,一种基于phi29DNA聚合酶滚环复制长链碱基修饰核酸活性制备水凝胶的方法,2021112052454,2025-06-27
- 陈庭坚;孙乐平;马杰钊;王雅鑫;王学婷;杜宇辉,一种无模板酶法合成含非天然碱基的核糖核酸链的方法及其应用,2024110863203,2025-02-07
- 陈庭坚;王光远;何传平;刘家韵,利用末端脱氧核糖核苷酸转移酶无模板合成含非天然碱基寡核苷酸链的方法及其应用,2021113025257,2023-09-29
- 陈庭坚;张汝洁;彭乐丽;叶方开;雷谨铭;何传平;王光远,一种非天然核酸水凝胶及其制备方法与应用,2021101013297,2023-08-18
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