个人简介
长期深耕CRISPR基因编辑与分子诊断领域前沿研究。围绕基因编辑机制、功能基因组学和即时诊断技术探索构建“基础机理探索-核心技术研发-产业应用转化”的完整创新体系。团队自主研发的CORDS生物传感技术平台，实现了从智能靶点设计、核心检测系统到终端产品的全链条技术突破，推动CRISPR检测技术从实验室走向实际应用。
坚持“授人以渔，知行合一”的育人理念。通过构建本硕博贯通的科研训练体系，指导学生深度参与前沿课题研究，培养学生扎实的科学素养和创新能力。指导学生多次获得国家级奖项与荣誉，毕业生已在学术界与产业界成为中坚力量。
研究成果荣获广东省科技进步一等奖、广州市科技进步一等奖等奖励，在Biosensors and Bioelectronics、Archives of Toxicology、ACS Sensors等国际权威期刊发表论文20余篇，授权发明专利8项（含国际专利1项）。未来，我将继续秉持“学高为师，身正为范”的初心，在CRISPR技术前沿“持续探险”，着力培养兼具国际视野、创新能力与社会担当的新一代生物医药交叉创新人才。
教育经历
2019-2020，新加坡国立大学，药学院，访问学者
2007-2010，南方医科大学/中科院广州生物医药与健康研究院，博士
2004-2007，南方医科大学/广州空军医院，硕士
2000-2004，江西农业大学，学士
招生信息
专业学位硕导，生物科学与工程学院，086000，生物与医药，生物工程
学术学位硕导，生物科学与工程学，,071000，生物学，生物化学与分子生物学
工作经历
- 2011.3~今,华南理工大学
- 2011-03,华南理工大学
社会、学会及学术兼职
研究领域
团队研究围绕CRISPR系统展开，致力于从基础机制探索到技术开发与转化的全链条创新，主要研究方向包括：
1. 基因编辑机制与应用：
聚焦CRISPR/Cas9系统的分子作用机理，旨在提升其编辑效率与靶向精准性，推动该技术在基因功能解析、疾病模型构建及基因治疗策略开发中的应用。
2. 高通量CRISPR功能基因组学：
建立基于CRISPR的高通量基因编辑与筛选平台，系统性揭示基因功能及其调控网络，支持疾病机制研究与创新药物靶点发现。
3. 分子诊断与生物传感：
利用CRISPR/Cas12/13系统开发开发新一代快速、高灵敏生物传感器，致力于实现病原微生物、基因变异及生物标志物的现场即时检测。
开授课程
本科生课程：《生理学》、《生理学实验》、《基因编辑前沿技术 》、《发育生物学》
研究生课程：《CRISPR/Cas基因编辑技术》、《现代生命科学研究进展》、《生命科学高等仪器原理及应用二》
科研项目
[1] 国家自然科学基金面上项目：CRISPR/CAS9诱导猪基因组损伤修复机制研究及其在精准基因编辑中的应用，2018-2022，主持
[2] 国家自然科学基金青年项目：piggyBac介导体外高灵敏药物代谢肝细胞模型的建立，2012–2015，主持。
[3] 国家重点研发计划“精准医学研究”重点专项：医学生命组学数据质量控制关键技术研发与应用示范，2018-2020，项目技术骨干
[4] 广东省自然科学基金项目：哺乳动物细胞内Cas13反式剪切活性的系统评价及胞内外差异机制探索，2022-2024，主持。
[5] 广东省自然科学基金项目：哺乳动物细胞内CRISPR/Cas9/dCas9基因编辑动力学特征研究，2019-2022，主持。
[6] 广东省科技计划项目：哺乳类实验动物SNP标记筛查的引物开发和推广应用，2015-2016，主持。
[7] 广州市科技计划重点研发项目：基于CRISPR/Cas12和靶序列自动筛选系统的非洲猪瘟病毒流行株及变异缺失株现场快速检测方法开发及推广，2022-2025，主持。
[8] 广东省药品监督管理局科技创新项目：医疗器械常见化学添加物皮肤致敏及刺激体外高通量细胞评价体系的研究，2024-2025，主持。
[9] 国家药品监督管理局重点实验室开放课题：皮肤致敏评价细胞模型 EndoSens 实验室间转移及验证研究，2024-2025，主持。
[10] 广东省医学科学技术项目：基于核酸识别的登革热病毒快速实时CRISPR/Cas检测技术研究，2019-2021，主持。
[11] 中央高校项目（2019MS089）：基于CRISPR/Cas13的核酸现场快速检测技术研究，2019.07-2020.12，主持。
[12] 中央高校项目：应用人源化小鼠研究双氯芬酸的肝毒性机制，2013-2015，主持。
[13] 中央高校项目：常用实验小型猪CYP3A体外表达体系的建立和代谢表征比较，2011-2012，主持。
[14] 科技合作开发项目：基于CORDS构建急性髓系白血病基因突变快速检测方法，2023-2024，主持。
[15] 科技合作开发项目：HBV靶向性CRISPR/Cas9系统的开发，2022-2025，主持。
[16] 科技合作开发项目：CRISPR/Cas介导药物转运体基因敲除细胞模型的开发构建，2022-2024，主持。
[17] 科技合作开发项目：DDX24基因点突变HUVEC细胞系建立，2018-2019，主持。
论文
- Han, Liya;Feng, Wenjun;Wang, Lv;Chen, Feng;Tan, Tianyi;Zhu, Yongchong;Li, Wenyan;Yang, Yunpeng;Xu, Wenyi;Li, Yue;Tang, Deyou;Hao, Lijing;Chen, Ruikang;Zheng, Qiaoyue;Mutanlifu, Zainaifu;Wan, Yanbin*;Chen, Tao*;Huang, Lizhen*,Smart-SNPer: Automated crRNA design for ultrasensitive RPA-Cas12a SNP detection,Biosensors and Bioelectronics,2025
- Wan, Yanbin;Huang, Caimei;Feng, Dongyan;Wang, Lv;Lin, Xiding;Zhao, Xuanye;Han, Liya;Zhu, Yanyu;Hao, Lijing;Du, Hongli;Huang, Lizhen*,Characterizing the collateral activity of CRISPR/Cas13 in mammalian cells: Implications for RNA editing and therapeutic applications,International Journal of Biological Macromolecules,2024
- Zhang Li;Wong Ling Rong;Wong Peiyan;Shen Wanxiang;Yang Shili;Huang Lizhen;Lim Yun-An;Ho Paul Chi-Lui,Chronic treatment with baicalein alleviates behavioural disorders and improves cerebral blood flow via reverting metabolic abnormalities in a J20 transgenic mouse model of Alzheimer's disease,Brain, Behavior, & Immunity - Health,2023
- Lin, Cailing;Chen, Feng;Huang, Dongchao;Li, Wenyan;He, Changsheng;Tang, Yingjun;Li, Xueping;Liu, Can;Han, Liya;Yang, Yunpeng;Zhu, Yongchong;Chen, Ruikang;Shi, Yuanju;Xia, Chenglai;Yan, Zhibin;Du, Hongli;Huang, Lizhen,A universal all-in-one RPA-Cas12a strategy with de novo autodesigner and its application in on-site ultrasensitive detection of DNA and RNA viruses,Biosensors and Bioelectronics,2023
- Xi Binbin;Zeng Xi;Chen Zixi;Zeng Jiong;Huang Lizhen;Du Hongli,SARS-CoV-2 within-host diversity of human hosts and its implications for viral immune evasion.,mBio,2023
- Chen, Hong;He, Bailin;Zhong, Suzhen;Huang, Dongchao;Deng, Shikai;Liu, Qifa;Li, Chengyao;Huang, Lizhen;Xu, Na,Disruption of SOCS1 Promotes the Antitumor Activity of CD19-Specific CAR NK Cells Coexpressing IL-15,Blood,2023
- Xu Wanqing;Zuo Qingxia;Feng Dongyan;He Changsheng;Lin Cailing;Huang Dongchao;Wan Yanbin;Chen Feng;Mo Guosheng;Sun Qi;Du Hongli;Huang Lizhen,Validation Study to Determine the Accuracy of Widespread Promoterless EGFP Reporter at Assessing CRISPR/Cas9-Mediated Homology Directed Repair,Current Issues in Molecular Biology,2022
- Xi, Binbin;Meng, Yuhuan;Jiang, Dawei;Bai, Yunmeng;Chen, Zixi;Qu, Yimo;Li, Shuhua;Wei, Jinfen;Huang, Lizhen;Du, Hongli,Analyses of Long-Term Epidemic Trends and Evolution Characteristics of Haplotype Subtypes Reveal the Dynamic Selection on SARS-CoV-2,Viruses-Basel,2022
- He Changsheng;Lin Cailing;Mo Guosheng;Xi Binbin;Li An′an;Huang Dongchao;Wan Yanbin;Chen Feng;Liang Yufeng;Zuo Qingxia;Xu Wanqing;Feng Dongyan;Zhang Guanting;Han Liya;Ke Changwen;Du Hongli;Huang Lizhen,Rapid and accurate detection of SARS-CoV-2 mutations using a Cas12a-based sensing platform,Biosensors and Bioelectronics,2022
- Zuo, Qingxia;Xu, Wanqing;Wan, Yanbin;Feng, Dongyan;He, Changsheng;Lin, Cailing;Huang, Dongchao;Chen, Feng;Han, Liya;Sun, Qi;Chen, Dong;Du, Hongli;Huang, Lizhen,Efficient generation of a CYP3A4-T2A-luciferase knock-in HepaRG subclone and its optimized differentiation,Biomedicine & Pharmacotherapy,2022
- Feng Dongyan;Zhong Guorui;Zuo Qingxia;Wan Yanbin;Xu Wanqing;He Changsheng;Lin Cailing;Huang Dongchao;Chen Feng;Huang Lizhen,Knockout of ABC transporters by CRISPR/Cas9 contributes to reliable and accurate transporter substrate identification for drug discovery,Frontiers in Pharmacology,2022
- Xi Binbin;Meng Yuhuan;Jiang Dawei;Bai Yunmeng;Chen Zixi;Qu Yimo;Li Shuhua;Wei Jinfen;Huang Lizhen;Du Hongli,Analyses of Long-Term Epidemic Trends and Evolution Characteristics of Haplotype Subtypes Reveal the Dynamic Selection on SARS-CoV-2,Viruses,2022
- Xi, Binbin;Chen, Zixi;Li, Shuhua;Liu, Wei;Jiang, Dawei;Bai, Yunmeng;Qu, Yimo;Lon, Jerome Rumdon;Huang, Lizhen;Du, Hongli*,AutoVEM2: A flexible automated tool to analyze candidate key mutations and epidemic trends for virus,Computational and Structural Biotechnology Journal,2021
- Xi Binbin;Jiang Dawei;Li Shuhua;Lon Jerome R;Bai Yunmeng;Lin Shudai;Hu Meiling;Meng Yuhuan;Qu Yimo;Huang Yuting;Liu Wei;Huang Lizhen;Du Hongli,AutoVEM: an automated tool to real-time monitor epidemic trends and key mutations in SARS-CoV-2 evolution,Computational and Structural Biotechnology Journal,2021
- Wang, Wei; Zhong, Yu; Zhuang, Zhenkun; Xie, Jiarui; Lu, Yueer; Huang, Chengzhi; Sun, Yan; Wu, Liang; Yin, Jianhua; Yu, Hang; Jiang, Zhiqiang; Wang, Shanshan; Wang, Chunqing; Zhang, Yuanhang; Huang, Yilin; Han, Chongyin; Zhong, Zhenggang; Hu, Jialin; Ouyang, Ying; Liu, Huisheng,Multiregion single-cell sequencing reveals the transcriptional landscape of the immune microenvironment of colorectal cancer,Clinical and Translational Medicine,2021
- Li Wenjing;Jiang Jingwei;Huang Lizhen;Long Feng,Efficacy of PD-1/L1 inhibitors in?brain metastases of non-small-cell lung cancer: pooled analysis from seven randomized controlled trials,Future Oncology,2021
- Dai Yu;Lim Jin Xuan;Yeo Samuel Chao Ming;Xiang Xiaoqiang;Tan Kai Soo;Fu Jia Hui;Huang Lizhen;Lin Hai-Shu,Biotransformation of Piceatannol, a Dietary Resveratrol Derivative: Promises to Human Health.,Molecular nutrition & food research,2020
- Jiang, Zhiqiang;Huang, Yilin;Zhang, Piao;Han, Chongyin;Lu, Yueer;Mo, Zongchao;Zhang, Zhanyu;Li, Xin;Zhao, Sisi;Cai, Fuqiang;Huang, Lizhen;Chen, Chunbo;Shi, Zhihong;Zhang, Yuhu;Ling, Fei,Characterization of a pathogenic variant in GBA for Parkinson's disease with mild cognitive impairment patients,Molecular Brain,2020
- Zhang, Li;Yang, Shili;Huang, Lizhen;Ho, Paul Chi-Lui,Poly (ethylene glycol)-block-poly (D, L-lactide) (PEG-PLA) micelles for brain delivery of baicalein through nasal route for potential treatment of neurodegenerative diseases due to oxidative stress and inflammation: An in vitro and in vivo study,International Journal of Pharmaceutics,2020
- Zhang, Wenlu;Bai, Yunmeng;Chen, Zixi;Li, Xingsong;Fu, Shuying;Huang, Lizhen;Lin, Shudai;Du, Hongli,Comprehensive analysis of long non-coding RNAs and mRNAs in skeletal muscle of diabetic Goto-Kakizaki rats during the early stage of type 2 diabetes,PeerJ,2020
- Weifan Jiang;Tianming Dai;Shuilin Xie;Lan Ding;Lizhen Huang;Renke Dai,Roles of diclofenac and its metabolites in immune activation associated with acute hepatotoxicity in TgCYP3A4/hPXR-humanized mice,International Immunopharmacology,2020
- Dai, Yu;Lim, Jin Xuan;Yeo, Samuel Chao Ming;Xiang, Xiaoqiang;Tan, Kai Soo;Fu, Jia Hui;Huang, Lizhen;Lin, Hai-Shu,Biotransformation of Piceatannol, a Dietary Resveratrol Derivative: Promises to Human Health,Molecular Nutrition & Food Research,2020
- Jiang, Weifan;Dai, Tianming;Xie, Shuilin;Ding, Lan;Huang, Lizhen*;Dai, Renke*,Roles of diclofenac and its metabolites in immune activation associated with acute hepatotoxicity in TgCYP3A4/hPXR-humanized mice,International Immunopharmacology,2020
- Li, Haojian;Bai, Jing;Zhong, Guorui;Lin, Haosi;He, Changsheng;Dai, Renke;Du, Hongli;Huang, Lizhen,Improved Defined Approaches for Predicting Skin Sensitization Hazard and Potency in Humans,Altex-Alternatives to Animal Experimentation,2019
- Fu, Shuying;Meng, Yuhuan;Lin, Shudai;Zhang, Wenlu;He, Yuting;Huang, Lizhen;Du, Hongli,Transcriptomic responses of hypothalamus to acute exercise in type 2 diabetic Goto-Kakizaki rats,PeerJ,2019
- Zhang, Wenlu;Meng, Yuhuan;Fu, Shuying;Li, Xingsong;Chen, Zixi;Huang, Lizhen;Du, Hongli,Transcriptome Changes of Skeletal Muscle RNA-Seq Speculates the Mechanism of Postprandial Hyperglycemia in Diabetic Goto-Kakizaki Rats During the Early Stage of T2D,Genes,2019
- Bai, Jing;Lin, Haosi;Li, Haojian;Zhou, Yang;Liu, Junshan;Zhong, Guorui;Wu, Luting;Jiang, Weifan;Du, Hongli;Yang, Jinyi;Xie, Qingmei;Huang, Lizhen,Cas12a-Based On-Site and Rapid Nucleic Acid Detection of African Swine Fever,Frontiers in Microbiology,2019
- Meng, Yuhuan;Cui, Ying;Zhang, Wenlu;Fu, Shuying;Huang, Lizhen;Dong, Hua;Du, Hongli,Integrative Analysis of Genome and Expression Profile Data Reveals the Genetic Mechanism of the Diabetic Pathogenesis in Goto Kakizaki (GK) Rats,Frontiers in Genetics,2019
- Fu, Shuying;Meng, Yuhuan;Zhang, Wenlu;Wang, Jiajian;He, Yuting;Huang, Lizhen;Chen, Hongmei;Kuang, Jian;Du, Hongli,Transcriptomic Responses of Skeletal Muscle to Acute Exercise in Diabetic Goto-Kakizaki Rats,Frontiers in Physiology,2019
- Lu, Yueer;Wang, Xiao;Yu, Hang;Li, Jianlin;Jiang, Zhiqiang;Chen, Bangwei;Lu, Yueqi;Wang, Wei;Han, Chongyin;Ouyang, Ying;Huang, Lizhen;Chen, Chunbo;Tian, Weidong;Ling, Fei,Evolution and Comprehensive Analysis of DNasel Hypersensitive Sites in Regulatory Regions of Primate Brain-Related Genes,Frontiers in Genetics,2019
- Jiang, Jingwei;Gao, Qingmin;Gong, Yiwei;Huang, Lizhen;Lin, Hao;Zhou, Xinli;Liang, Xiaohua;Guo, Weijian,MiR-486 promotes proliferation and suppresses apoptosis in myeloid cells by targeting Cebpa in vitro,Cancer Medicine,2018
- Pang S.-H.;Zhong G.-R.;Xie S.-L.;Li H.-J.;Zou S.-X.;Jia Y.;Dai R.-K.;Huang L.-Z.*,Comparison of methods for stable co-expression of different subtype drug-matabolizing enzymes in HepG2 cells by piggyBac transposon,Chinese Journal of Pharmacology and Toxicology,2018
- Yu, Xiaokang;Liang, Jinsheng;Xu, Jiarui;Li, Xingsong;Xing, Shan;Li, Huilan;Liu, Wanli;Liu, Dongdong;Xu, Jianhua;Huang, Lizhen;Du, Hongli,Identification and Validation of Circulating MicroRNA Signatures for Breast Cancer Early Detection Based on Large Scale Tissue-Derived Data,Journal of Breast Cancer,2018
- Zhong, Guorui;Li, Haojian;Bai, Jing;Pang, Shihui;He, Changsheng;Du, Xinyi;Wang, Haijie;Zhang, Qixiao;Xie, Shuilin;Du, Hongli;Dai, Renke;Huang, Lizhen,Advancing the predictivity of skin sensitization by applying a novel HMOX1 reporter system,Archives of Toxicology,2018
- Li, Xingsong;Yu, Xiaokang;He, Yuting;Meng, Yuhuan;Liang, Jinsheng;Huang, Lizhen;Du, Hongli;Wang, Xueping;Liu, Wanli,Integrated Analysis of MicroRNA (miRNA) and mRNA Profiles Reveals Reduced Correlation between MicroRNA and Target Gene in Cancer,Biomed Research International,2018
出版专著和教材
- 猪场疫病检测技术,广东科技出版社,2023-06
专利
- 黄黎珍;钟国瑞;李浩健;谢水林;庞士慧;白静;何昌生;都心怡;张琪霄;王海杰;杜红丽,一种化合物皮肤致敏体外评估细胞模型及其构建方法,US 12,173,283 B2,2024-12-24 00:00:00
- 黄黎珍;林采玲;武鹭婷;何昌生;莫国圣;许万青;左青霞;冯冬燕,一种基于CRISPR/Cas12a和恒温扩增的DENV一步法核酸检测方法,2021104587293,2024-02-20
- 黄黎珍;何昌生;莫国圣;杜红丽;林采玲;万延斌;武鹭婷;左青霞;冯东燕;许万青;孙琪;谢震燊;庄镇豪,基于CRISPR/Cas12a技术快速检测新冠病毒和突变株的方法及试剂盒,2021105898228,2024-01-09
- 黄黎珍;陈锋;林采玲;李文艳;韩立亚;陈睿康;杨云鹏,一种基于单管PRA-CRISPR/Cas12a的现场便携式检测装置,2023202406616,2023-12-15
- 黄黎珍;林浩斯;钟国瑞;李浩健;杜红丽;白静;何昌生;谢水林;戴仁科,一种用于检测PRRSV的现场快速检测方法及试剂盒,2018107956495,2020-09-22
- 黄黎珍;钟国瑞;李浩健;谢水林;庞士慧;白静;何昌生;都心怡;张琪霄;王海杰;杜红丽,一种化合物皮肤致敏体外评估细胞模型及其构建方法,2017113354633,2020-07-28
- 黄黎珍;林浩斯;白静;周阳;武鹭婷;汪凯婷;江伟凡;白云梦;杜红丽,一种基于CRISPR/Cas系统用于检测ASFV的现场快速检测方法及试剂盒,2019104673395,2020-07-28
- 黄黎珍;李文艳;朱泳冲;万延斌;林境烁,一种现场快速鉴别非洲猪瘟野毒株与基因缺失疫苗株的检测组合物、试剂盒及方法,202510067038
获奖、荣誉称号
- 广东省科技进步奖,生物科学与工程学院,黄黎珍,2016-02-01,省部级,一等奖
- 广州市科技进步奖,生物科学与工程学院,黄黎珍,2015-05-25,厅局级,一等奖
- 溢多利奖教金,生物科学与工程学院,黄黎珍,2024-09,教学成果奖
- 2023-2024学年度本科生优秀班主任,生物科学与工程学院,黄黎珍,2024-07,教学优秀奖
- 2019届本科毕业设计（论文）优秀指导教师奖,生物科学与工程学院,黄黎珍,2019
- 2018学年度本科毕业设计（论文）优秀指导教师奖,生物科学与工程学院,黄黎珍,2018学年度,本科毕业设计（论文）优秀指导教师奖
- 2016年学生科技创新优秀指导教师三等奖,生物科学与工程学院,黄黎珍,2016年,学生科技创新优秀指导教师三等奖
- 2015学年度本科毕业设计（论文）优秀指导教师奖,生物科学与工程学院,黄黎珍,2015学年度,本科毕业设计（论文）优秀指导教师奖
- 2014-2015学年青年教师课堂教学竞赛鼓励奖,生物科学与工程学院,黄黎珍,2014-2015学年,青年教师课堂教学竞赛鼓励奖
- 第十六届“挑战杯”广东大学生课外学术科技作品竞赛,生物科学与工程学院,邱若然、张思清、李淑桦、张芯语、谭佳宜、莫国圣、蔡富强、席彬彬、龙润东,省级（含区域赛）,一等奖
软著
- RPA-Cas12a单管一体化检测的crRNA及引物自动化设计软件[简称：AutoCORDS]V1.0,软著登字第11403962号,2023SR0816791,2023-07-06 00:00:00
- CORDS单碱基突变检测crRNA及引物自动化设计软件[简称：Smart-SNPer]V1.0,软著登字第14267047号,2024SR1863174,2024-11-22 00:00:00
- 用于Repair-seq实验平台的一体化数据分析及绘图软件[简称：WIICORepair-seq]V1.0,软著登字第12789961号,2024SR0386088,2024-03-13 00:00:00
- CRISPR抗病毒靶点自动化设计软件[简称：AutoViroCRISPR]V1.0,软著登字第16810419号,2025SR2154221,2025-11-05 00:00:00