Biography
Education
Admission Information
Work Experience
Social Position
Research Areas
Our research team focuses on the CRISPR system, dedicated to achieving full-chain innovation from exploring fundamental mechanisms to technology development and translation. The main research directions include:
Gene Editing Mechanisms and Applications
Concentrating on the molecular mechanisms of the CRISPR/Cas9 system, we aim to enhance its editing efficiency and targeting precision, thereby advancing its applications in gene function analysis, disease modeling, and the development of gene therapy strategies.High-Throughput CRISPR Functional Genomics
We are establishing a CRISPR-based high-throughput gene editing and screening platform to systematically uncover gene functions and their regulatory networks, supporting disease mechanism studies and the discovery of innovative drug targets.Molecular Diagnostics and Biosensing
Leveraging the CRISPR/Cas12/13 systems, we are developing a new generation of rapid and highly sensitive biosensors, striving to achieve on-site, real-time detection of pathogenic microorganisms, genetic variants, and biomarkers.
Students interested in CRISPR/Cas systems are encouraged to reach out to explore research opportunities in areas such as gene editing mechanisms, high-throughput functional genomics, and CRISPR-based molecular diagnostics.
Courses Taught
Undergraduate Courses:
Physiology
Physiology Laboratory
Frontiers in Gene Editing Technology
Developmental Biology
Graduate Courses:
CRISPR/Cas Gene Editing Technology
Advances in Modern Life Sciences Research
Principles and Applications of Advanced Instruments in Life Sciences II
Research Project
Selected Publications
- Han, Liya;Feng, Wenjun;Wang, Lv;Chen, Feng;Tan, Tianyi;Zhu, Yongchong;Li, Wenyan;Yang, Yunpeng;Xu, Wenyi;Li, Yue;Tang, Deyou;Hao, Lijing;Chen, Ruikang;Zheng, Qiaoyue;Mutanlifu, Zainaifu;Wan, Yanbin*;Chen, Tao*;Huang, Lizhen*,Smart-SNPer: Automated crRNA design for ultrasensitive RPA-Cas12a SNP detection,Biosensors and Bioelectronics,2025
- Wan, Yanbin;Huang, Caimei;Feng, Dongyan;Wang, Lv;Lin, Xiding;Zhao, Xuanye;Han, Liya;Zhu, Yanyu;Hao, Lijing;Du, Hongli;Huang, Lizhen*,Characterizing the collateral activity of CRISPR/Cas13 in mammalian cells: Implications for RNA editing and therapeutic applications,International Journal of Biological Macromolecules,2024
- Zhang Li;Wong Ling Rong;Wong Peiyan;Shen Wanxiang;Yang Shili;Huang Lizhen;Lim Yun-An;Ho Paul Chi-Lui,Chronic treatment with baicalein alleviates behavioural disorders and improves cerebral blood flow via reverting metabolic abnormalities in a J20 transgenic mouse model of Alzheimer's disease,Brain, Behavior, & Immunity - Health,2023
- Lin, Cailing;Chen, Feng;Huang, Dongchao;Li, Wenyan;He, Changsheng;Tang, Yingjun;Li, Xueping;Liu, Can;Han, Liya;Yang, Yunpeng;Zhu, Yongchong;Chen, Ruikang;Shi, Yuanju;Xia, Chenglai;Yan, Zhibin;Du, Hongli;Huang, Lizhen,A universal all-in-one RPA-Cas12a strategy with de novo autodesigner and its application in on-site ultrasensitive detection of DNA and RNA viruses,Biosensors and Bioelectronics,2023
- Xi Binbin;Zeng Xi;Chen Zixi;Zeng Jiong;Huang Lizhen;Du Hongli,SARS-CoV-2 within-host diversity of human hosts and its implications for viral immune evasion.,mBio,2023
- Chen, Hong;He, Bailin;Zhong, Suzhen;Huang, Dongchao;Deng, Shikai;Liu, Qifa;Li, Chengyao;Huang, Lizhen;Xu, Na,Disruption of SOCS1 Promotes the Antitumor Activity of CD19-Specific CAR NK Cells Coexpressing IL-15,Blood,2023
- Xu Wanqing;Zuo Qingxia;Feng Dongyan;He Changsheng;Lin Cailing;Huang Dongchao;Wan Yanbin;Chen Feng;Mo Guosheng;Sun Qi;Du Hongli;Huang Lizhen,Validation Study to Determine the Accuracy of Widespread Promoterless EGFP Reporter at Assessing CRISPR/Cas9-Mediated Homology Directed Repair,Current Issues in Molecular Biology,2022
- Xi, Binbin;Meng, Yuhuan;Jiang, Dawei;Bai, Yunmeng;Chen, Zixi;Qu, Yimo;Li, Shuhua;Wei, Jinfen;Huang, Lizhen;Du, Hongli,Analyses of Long-Term Epidemic Trends and Evolution Characteristics of Haplotype Subtypes Reveal the Dynamic Selection on SARS-CoV-2,Viruses-Basel,2022
- He Changsheng;Lin Cailing;Mo Guosheng;Xi Binbin;Li An′an;Huang Dongchao;Wan Yanbin;Chen Feng;Liang Yufeng;Zuo Qingxia;Xu Wanqing;Feng Dongyan;Zhang Guanting;Han Liya;Ke Changwen;Du Hongli;Huang Lizhen,Rapid and accurate detection of SARS-CoV-2 mutations using a Cas12a-based sensing platform,Biosensors and Bioelectronics,2022
- Zuo, Qingxia;Xu, Wanqing;Wan, Yanbin;Feng, Dongyan;He, Changsheng;Lin, Cailing;Huang, Dongchao;Chen, Feng;Han, Liya;Sun, Qi;Chen, Dong;Du, Hongli;Huang, Lizhen,Efficient generation of a CYP3A4-T2A-luciferase knock-in HepaRG subclone and its optimized differentiation,Biomedicine & Pharmacotherapy,2022
- Feng Dongyan;Zhong Guorui;Zuo Qingxia;Wan Yanbin;Xu Wanqing;He Changsheng;Lin Cailing;Huang Dongchao;Chen Feng;Huang Lizhen,Knockout of ABC transporters by CRISPR/Cas9 contributes to reliable and accurate transporter substrate identification for drug discovery,Frontiers in Pharmacology,2022
- Xi Binbin;Meng Yuhuan;Jiang Dawei;Bai Yunmeng;Chen Zixi;Qu Yimo;Li Shuhua;Wei Jinfen;Huang Lizhen;Du Hongli,Analyses of Long-Term Epidemic Trends and Evolution Characteristics of Haplotype Subtypes Reveal the Dynamic Selection on SARS-CoV-2,Viruses,2022
- Xi, Binbin;Chen, Zixi;Li, Shuhua;Liu, Wei;Jiang, Dawei;Bai, Yunmeng;Qu, Yimo;Lon, Jerome Rumdon;Huang, Lizhen;Du, Hongli*,AutoVEM2: A flexible automated tool to analyze candidate key mutations and epidemic trends for virus,Computational and Structural Biotechnology Journal,2021
- Xi Binbin;Jiang Dawei;Li Shuhua;Lon Jerome R;Bai Yunmeng;Lin Shudai;Hu Meiling;Meng Yuhuan;Qu Yimo;Huang Yuting;Liu Wei;Huang Lizhen;Du Hongli,AutoVEM: an automated tool to real-time monitor epidemic trends and key mutations in SARS-CoV-2 evolution,Computational and Structural Biotechnology Journal,2021
- Wang, Wei; Zhong, Yu; Zhuang, Zhenkun; Xie, Jiarui; Lu, Yueer; Huang, Chengzhi; Sun, Yan; Wu, Liang; Yin, Jianhua; Yu, Hang; Jiang, Zhiqiang; Wang, Shanshan; Wang, Chunqing; Zhang, Yuanhang; Huang, Yilin; Han, Chongyin; Zhong, Zhenggang; Hu, Jialin; Ouyang, Ying; Liu, Huisheng,Multiregion single-cell sequencing reveals the transcriptional landscape of the immune microenvironment of colorectal cancer,Clinical and Translational Medicine,2021
- Li Wenjing;Jiang Jingwei;Huang Lizhen;Long Feng,Efficacy of PD-1/L1 inhibitors in?brain metastases of non-small-cell lung cancer: pooled analysis from seven randomized controlled trials,Future Oncology,2021
- Dai Yu;Lim Jin Xuan;Yeo Samuel Chao Ming;Xiang Xiaoqiang;Tan Kai Soo;Fu Jia Hui;Huang Lizhen;Lin Hai-Shu,Biotransformation of Piceatannol, a Dietary Resveratrol Derivative: Promises to Human Health.,Molecular nutrition & food research,2020
- Jiang, Zhiqiang;Huang, Yilin;Zhang, Piao;Han, Chongyin;Lu, Yueer;Mo, Zongchao;Zhang, Zhanyu;Li, Xin;Zhao, Sisi;Cai, Fuqiang;Huang, Lizhen;Chen, Chunbo;Shi, Zhihong;Zhang, Yuhu;Ling, Fei,Characterization of a pathogenic variant in GBA for Parkinson's disease with mild cognitive impairment patients,Molecular Brain,2020
- Zhang, Li;Yang, Shili;Huang, Lizhen;Ho, Paul Chi-Lui,Poly (ethylene glycol)-block-poly (D, L-lactide) (PEG-PLA) micelles for brain delivery of baicalein through nasal route for potential treatment of neurodegenerative diseases due to oxidative stress and inflammation: An in vitro and in vivo study,International Journal of Pharmaceutics,2020
- Zhang, Wenlu;Bai, Yunmeng;Chen, Zixi;Li, Xingsong;Fu, Shuying;Huang, Lizhen;Lin, Shudai;Du, Hongli,Comprehensive analysis of long non-coding RNAs and mRNAs in skeletal muscle of diabetic Goto-Kakizaki rats during the early stage of type 2 diabetes,PeerJ,2020
- Weifan Jiang;Tianming Dai;Shuilin Xie;Lan Ding;Lizhen Huang;Renke Dai,Roles of diclofenac and its metabolites in immune activation associated with acute hepatotoxicity in TgCYP3A4/hPXR-humanized mice,International Immunopharmacology,2020
- Dai, Yu;Lim, Jin Xuan;Yeo, Samuel Chao Ming;Xiang, Xiaoqiang;Tan, Kai Soo;Fu, Jia Hui;Huang, Lizhen;Lin, Hai-Shu,Biotransformation of Piceatannol, a Dietary Resveratrol Derivative: Promises to Human Health,Molecular Nutrition & Food Research,2020
- Jiang, Weifan;Dai, Tianming;Xie, Shuilin;Ding, Lan;Huang, Lizhen*;Dai, Renke*,Roles of diclofenac and its metabolites in immune activation associated with acute hepatotoxicity in TgCYP3A4/hPXR-humanized mice,International Immunopharmacology,2020
- Li, Haojian;Bai, Jing;Zhong, Guorui;Lin, Haosi;He, Changsheng;Dai, Renke;Du, Hongli;Huang, Lizhen,Improved Defined Approaches for Predicting Skin Sensitization Hazard and Potency in Humans,Altex-Alternatives to Animal Experimentation,2019
- Fu, Shuying;Meng, Yuhuan;Lin, Shudai;Zhang, Wenlu;He, Yuting;Huang, Lizhen;Du, Hongli,Transcriptomic responses of hypothalamus to acute exercise in type 2 diabetic Goto-Kakizaki rats,PeerJ,2019
- Zhang, Wenlu;Meng, Yuhuan;Fu, Shuying;Li, Xingsong;Chen, Zixi;Huang, Lizhen;Du, Hongli,Transcriptome Changes of Skeletal Muscle RNA-Seq Speculates the Mechanism of Postprandial Hyperglycemia in Diabetic Goto-Kakizaki Rats During the Early Stage of T2D,Genes,2019
- Bai, Jing;Lin, Haosi;Li, Haojian;Zhou, Yang;Liu, Junshan;Zhong, Guorui;Wu, Luting;Jiang, Weifan;Du, Hongli;Yang, Jinyi;Xie, Qingmei;Huang, Lizhen,Cas12a-Based On-Site and Rapid Nucleic Acid Detection of African Swine Fever,Frontiers in Microbiology,2019
- Meng, Yuhuan;Cui, Ying;Zhang, Wenlu;Fu, Shuying;Huang, Lizhen;Dong, Hua;Du, Hongli,Integrative Analysis of Genome and Expression Profile Data Reveals the Genetic Mechanism of the Diabetic Pathogenesis in Goto Kakizaki (GK) Rats,Frontiers in Genetics,2019
- Fu, Shuying;Meng, Yuhuan;Zhang, Wenlu;Wang, Jiajian;He, Yuting;Huang, Lizhen;Chen, Hongmei;Kuang, Jian;Du, Hongli,Transcriptomic Responses of Skeletal Muscle to Acute Exercise in Diabetic Goto-Kakizaki Rats,Frontiers in Physiology,2019
- Lu, Yueer;Wang, Xiao;Yu, Hang;Li, Jianlin;Jiang, Zhiqiang;Chen, Bangwei;Lu, Yueqi;Wang, Wei;Han, Chongyin;Ouyang, Ying;Huang, Lizhen;Chen, Chunbo;Tian, Weidong;Ling, Fei,Evolution and Comprehensive Analysis of DNasel Hypersensitive Sites in Regulatory Regions of Primate Brain-Related Genes,Frontiers in Genetics,2019
- Jiang, Jingwei;Gao, Qingmin;Gong, Yiwei;Huang, Lizhen;Lin, Hao;Zhou, Xinli;Liang, Xiaohua;Guo, Weijian,MiR-486 promotes proliferation and suppresses apoptosis in myeloid cells by targeting Cebpa in vitro,Cancer Medicine,2018
- Pang S.-H.;Zhong G.-R.;Xie S.-L.;Li H.-J.;Zou S.-X.;Jia Y.;Dai R.-K.;Huang L.-Z.*,Comparison of methods for stable co-expression of different subtype drug-matabolizing enzymes in HepG2 cells by piggyBac transposon,Chinese Journal of Pharmacology and Toxicology,2018
- Yu, Xiaokang;Liang, Jinsheng;Xu, Jiarui;Li, Xingsong;Xing, Shan;Li, Huilan;Liu, Wanli;Liu, Dongdong;Xu, Jianhua;Huang, Lizhen;Du, Hongli,Identification and Validation of Circulating MicroRNA Signatures for Breast Cancer Early Detection Based on Large Scale Tissue-Derived Data,Journal of Breast Cancer,2018
- Zhong, Guorui;Li, Haojian;Bai, Jing;Pang, Shihui;He, Changsheng;Du, Xinyi;Wang, Haijie;Zhang, Qixiao;Xie, Shuilin;Du, Hongli;Dai, Renke;Huang, Lizhen,Advancing the predictivity of skin sensitization by applying a novel HMOX1 reporter system,Archives of Toxicology,2018
- Li, Xingsong;Yu, Xiaokang;He, Yuting;Meng, Yuhuan;Liang, Jinsheng;Huang, Lizhen;Du, Hongli;Wang, Xueping;Liu, Wanli,Integrated Analysis of MicroRNA (miRNA) and mRNA Profiles Reveals Reduced Correlation between MicroRNA and Target Gene in Cancer,Biomed Research International,2018
Achievements
Patent
Honor
Software achievement